goat anti mouse igg antibody conjugated to cy3  (Jackson Immuno)

Journal: Non-coding RNA Research

Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development

doi: 10.1016/j.ncrna.2026.01.004

Figure Lengend Snippet: DPC-Exos transported miR-218-5p from DPCs to HFSCs. (A) Fluorescence imaging of HFSCs after treatment with DiI-labeled DPC-Exos (scale bar = 50 μm). (B) Schematic of the DPC–HFSC co-culture system with fluorescence showing intercellular transfer of exosomal miR-218–5p. (C) MiR-218–5p expression in HFSCs after transfection with Cy3-miR-218–5p (unpaired two-tailed t -test, n = 3). (D) MiR-218–5p expression in HFSCs after DPCs were transfected with siRNA-Drosha (unpaired two-tailed t -test, n = 3). (E) MiR-218–5p expression in HFSCs after DPCs were treated with GW4869 (unpaired two-tailed t -test, n = 3). ∗∗ P < 0.01.

Article Snippet: After three times washing with PBS containing 0.1 % Tween-20 (Solarbio, China, Cat. No. ST825), secondary antibodies Cy3-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00009-1) and Fluorescein (FlTc)-conjugated Goat Anti-Mouse lgG (Proteintech, China, Cat No. SA00003-1) were applied for incubation.

Techniques: Fluorescence, Imaging, Labeling, Co-Culture Assay, Expressing, Transfection, Two Tailed Test

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Low Prevalence and Inconsistency of LRP4-IgG Detection in Suspected Myasthenia Gravis

doi: 10.1212/NXI.0000000000200554

Figure Lengend Snippet: Three protocols of CBA were performed: l-CBA (A–C), pf-CBA (D–F), and mf-CBA (G–I). 4′,6-diamidino-2-phenylindole (DAPI) (blue channel) shows nuclear staining, and green fluorescent protein (GFP) expression (green channel) indicates transfected cells. For each protocol, LRP4 antibody was visualized with Cy3 anti-mouse IgG (red fluorescence). CBA = cell-based assay; LRP4-IgG = lipoprotein receptor-related protein 4; mf-CBA = methanol-fixed CBA; pf-CBA = paraformaldehyde-fixed CBA.

Article Snippet: For its detection, Cy3 AffiniPure Donkey Anti-Mouse IgG (H + L) (Jackson Immuno Research #715-165-151) was used as the secondary antibody (1:1000).

Techniques: Staining, Expressing, Transfection, Fluorescence, Cell Based Assay

Journal: Neural Regeneration Research

Article Title: Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr 9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma model

doi: 10.4103/NRR.NRR-D-24-00461

Figure Lengend Snippet: Effects of Kir4.1 overexpression and Kir4.1Tyr 9 Asp overexpression on GFAP expression in cultured Müller glial cells. (A, B) Representative immunoblots (A) and densitometric quantification (B) showing changes in GFAP expression in non-treated and DHPG-treated cultured Müller cells infected with lentiviruses overexpressing the eGFP control (LV-NC), Kir4.1, and Kir4.1 Tyr 9 Asp. All data are normalized to GADPH and then to Ctr. One-way analysis of variance (Tukey–Kramer multiple comparisons test) was performed (** P < 0.01, vs . Ctr group). (C) Double immunofluorescence staining showing changes in GFAP (Cy3, red) expression in non-treated (c1–c12) and DHPG-treated (c13–24) cultured Müller cells infected with lentiviruses overexpressing the eGFP Ctr (LV-NC) (c4–c6 and c16–c18), Kir4.1 (c7–c9 and c19–c21), and Kir4.1 Tyr 9 Asp (c10–c12 and c22–c24) mutation. Kir4.1 overexpression and Kir4.1 Tyr 9 Asp overexpression reduced the DHPG-induced increase in GFAP expression in Müller cells. GFP (indicated by Alexa488) and DAPI are shown in green and blue, respectively. Scale bars: 20 µm. Ctr: Control; DHPG: (S)-3,5-dihydroxyphenylglycine; eGFP: enhanced green fluorescent protein; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein; LV-NC: eGFP control lentiviruses.

Article Snippet: The secondary antibodies used were donkey anti-mouse Cy3 (1:500, Cat# 715-165-151, RRID: AB_2315777) and donkey anti-rabbit 488 (1:500, Cat# 711-545-152, RRID: AB_2313584, Jackson ImmunoResearch Labs).

Techniques: Over Expression, Expressing, Cell Culture, Western Blot, Infection, Control, Double Immunofluorescence Staining, Mutagenesis